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rabbit anti pkm2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit anti pkm2
    Rabbit Anti Pkm2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 745 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+pkm2/PKM2+XP+Rabbit+mAb/pmc12808913-87-46-49
    Average 96 stars, based on 745 article reviews
    rabbit anti pkm2 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Depression exacerbates AD pathology through lactate-dependent activation of microglial Kv1.3 to promote Aβ-containing exosome spreading.
    Article Snippet: .. Cells were blocked with 5% BSA for 1 h at room temperature and then incubated with primary antibodies including: Goat anti-Iba1(Novus, NB100-1028, 1:1000), Guinea pig anti-Kv1.3 (Alomone, AGP-005, 1:1000), Rabbit anti-PKM2 (Cell Signaling Technology, 4053, 1:1000), Rabbit anti-TSG101 (Proteintech, 28283-1-AP, 1:500), Mouse anti-β-Amyloid (Biolegend, 803014, 1:2000) overnight at 4 °C. .. Cells were then rinsed with PBS and incubated with fluorescenceconjugated secondary antibodies including: Donkey antiGoat 488 (Jackson, 705-545-147), Donkey anti-Guinea pig 647 (Jackson, 706-605-148), Donkey anti-Rabbit 488 (Jackson,711-545-152), Donkey anti-Mouse 555 (Bioss, bs-0295D-BF555), diluted 1:1000 in blocking buffer for 1 h at room temperature.

    Article Title: Oxidized LDL stimulates PKM2-mediated mtROS production and phagocytosis
    Article Snippet: .. Macrophages were fixed and incubated with rabbit anti-PKM2 (Cell Signaling, Cat4053) and mouse anti-UQCRC1 monoclonal antibodies (Invitrogen, Cat#459140). ..

    Article Title: Depression exacerbates AD pathology through lactate-dependent activation of microglial Kv1.3 to promote Aβ-containing exosome spreading
    Article Snippet: .. Cells were blocked with 5% BSA for 1 h at room temperature and then incubated with primary antibodies including: Goat anti-Iba1(Novus, NB100-1028, 1:1000), Guinea pig anti-Kv1.3 (Alomone, AGP-005, 1:1000), Rabbit anti-PKM2 (Cell Signaling Technology, 4053, 1:1000), Rabbit anti-TSG101 (Proteintech, 28283-1-AP, 1:500), Mouse anti-β-Amyloid (Biolegend, 803014, 1:2000) overnight at 4 °C. .. Cells were then rinsed with PBS and incubated with fluorescence-conjugated secondary antibodies including: Donkey anti-Goat 488 (Jackson, 705-545-147), Donkey anti-Guinea pig 647 (Jackson, 706-605-148), Donkey anti-Rabbit 488 (Jackson,711-545-152), Donkey anti-Mouse 555 (Bioss, bs-0295D-BF555), diluted 1:1000 in blocking buffer for 1 h at room temperature.

    Article Title: Depression exacerbates AD pathology through lactate-dependent activation of microglial Kv1.3 to promote Aβ-containing exosome spreading
    Article Snippet: Brain sections (~ 70 μm) were prepared using a freezing microtome (Leica, Germany, CM1950) and stored at -80 °C. .. The frozen brain sections or the recorded brain slices in electrophysiology were incubated in blocking solution (10% normal donkey serum, 5% BSA and 0.3% Triton X-100 in PBS) for ~ 2 h at room temperature, and then incubated with primary antibodies including: Rabbit anti-Iba1 (Wako, 019-19741, 1:1000), Goat anti-Iba1 (Novus, NB100-1028, 1:1000), Mouse anti-Kv1.3 (Santa, sc-398855, 1:2000), Mouse anti-β-Amyloid (Biolegend, 803014, 1:2000), Rabbit anti-PKM2 (Cell Signaling Technology, 4053, 1:500), Rabbit anti-TSG101 (proteintech, 28283-1-AP, 1:500) for 24 h at 4 °C. .. Brain slices were rinsed 3 times for 10 min with PBS and incubated with appropriate fluorescence-conjugated secondary antibodies, including: Donkey anti-Goat 488 (Jackson, 705-545-147), Donkey anti-Rabbit 488 (Jackson,711-545-152), Donkey anti-Mouse 555 (Bioss, bs-0295D-BF555) for 2 h at room temperature.

    Article Title: Bioengineered extracellular vesicles escape lysosomal degradation and deliver Tet-PKM2 for macrophage immunometabolic reprogramming and periodontitis treatment
    Article Snippet: The gingival and decalcified tissues were subsequently paraffin-embedded, cut into 2-μm-thick sections using a microtome (Leica), deparaffinized and subjected to antigen retrieval using 10 mM sodium citrate buffer. .. The tissue sections were subsequently permeabilized and blocked with 5 % bovine serum albumin (BSA; Beyotime, China) for 1 h at room temperature, after which each section was incubated with the following primary antibodies overnight at 4 °C: mouse anti-CD68 (1:200, Santa Cruz, SC-20060, USA) plus rabbit anti-PKM2 (1:200, Cell Signaling Technology, 4053S, USA), mouse anti-CD68 (1:200, Santa Cruz, SC-20060) plus rabbit anti-iNOS (1:200, Proteintech, 80517-1-RR, China), mouse anti-CD68 plus rabbit anti-CCR7 (1:200, Abcam, ab32527, UK), mouse anti-CD68 plus rabbit anti-CD163 (1:200, Proteintech, 16646-1-AP), mouse anti-CD68 plus rabbit anti-CD206 (1:200, Abcam, ab64693), or mouse anti-CD68 plus rabbit anti-Hif-1α (1:300, Cell Signaling Technology, 36169T). .. After being rinsed with PBS, the sections were incubated with the following corresponding secondary antibodies for 1 h at room temperature: donkey anti-rabbit IgG Alexa Fluor 488 (1:500; Abbkine, A23220, China) and donkey anti-mouse IgG Alexa Fluor 594 (1:500; Abbkine, A23410).

    Article Title: Depression exacerbates AD pathology through lactate-dependent activation of microglial Kv1.3 to promote Aβ-containing exosome spreading.
    Article Snippet: Brain sections (~ 70 μm) were prepared using a freezing microtome (Leica, Germany, CM1950) and stored at -80 °C. .. The frozen brain sections or the recorded brain slices in electrophysiology were incubated in blocking solution (10% normal donkey serum, 5% BSA and 0.3% Triton X-100 in PBS) for ~ 2 h at room temperature, and then incubated with primary antibodies including: Rabbit anti-Iba1 (Wako, 019-19741, 1:1000), Goat anti-Iba1 (Novus, NB100-1028, 1:1000), Mouse anti-Kv1.3 (Santa, sc-398855, 1:2000), Mouse anti-β-Amyloid (Biolegend, 803014, 1:2000), Rabbit anti-PKM2 (Cell Signaling Technology, 4053, 1:500), Rabbit anti-TSG101 (proteintech, 28283-1-AP, 1:500) for 24 h at 4 °C. .. Brain slices were rinsed 3 times for 10 min with PBS and incubated with appropriate fluorescence-conjugated secondary antibodies, including: Donkey anti-Goat 488 (Jackson, 705-545- 147), Donkey anti-Rabbit 488 (Jackson,711-545-152), Donkey anti-Mouse 555 (Bioss, bs-0295D-BF555) for 2 h at room temperature.

    Bioprocessing:

    Article Title: Oxidized LDL stimulates PKM2-mediated mtROS production and phagocytosis
    Article Snippet: .. Macrophages were fixed and incubated with rabbit anti-PKM2 (Cell Signaling, Cat4053) and mouse anti-UQCRC1 monoclonal antibodies (Invitrogen, Cat#459140). ..

    Blocking Assay:

    Article Title: Depression exacerbates AD pathology through lactate-dependent activation of microglial Kv1.3 to promote Aβ-containing exosome spreading
    Article Snippet: Brain sections (~ 70 μm) were prepared using a freezing microtome (Leica, Germany, CM1950) and stored at -80 °C. .. The frozen brain sections or the recorded brain slices in electrophysiology were incubated in blocking solution (10% normal donkey serum, 5% BSA and 0.3% Triton X-100 in PBS) for ~ 2 h at room temperature, and then incubated with primary antibodies including: Rabbit anti-Iba1 (Wako, 019-19741, 1:1000), Goat anti-Iba1 (Novus, NB100-1028, 1:1000), Mouse anti-Kv1.3 (Santa, sc-398855, 1:2000), Mouse anti-β-Amyloid (Biolegend, 803014, 1:2000), Rabbit anti-PKM2 (Cell Signaling Technology, 4053, 1:500), Rabbit anti-TSG101 (proteintech, 28283-1-AP, 1:500) for 24 h at 4 °C. .. Brain slices were rinsed 3 times for 10 min with PBS and incubated with appropriate fluorescence-conjugated secondary antibodies, including: Donkey anti-Goat 488 (Jackson, 705-545-147), Donkey anti-Rabbit 488 (Jackson,711-545-152), Donkey anti-Mouse 555 (Bioss, bs-0295D-BF555) for 2 h at room temperature.

    Article Title: Depression exacerbates AD pathology through lactate-dependent activation of microglial Kv1.3 to promote Aβ-containing exosome spreading.
    Article Snippet: Brain sections (~ 70 μm) were prepared using a freezing microtome (Leica, Germany, CM1950) and stored at -80 °C. .. The frozen brain sections or the recorded brain slices in electrophysiology were incubated in blocking solution (10% normal donkey serum, 5% BSA and 0.3% Triton X-100 in PBS) for ~ 2 h at room temperature, and then incubated with primary antibodies including: Rabbit anti-Iba1 (Wako, 019-19741, 1:1000), Goat anti-Iba1 (Novus, NB100-1028, 1:1000), Mouse anti-Kv1.3 (Santa, sc-398855, 1:2000), Mouse anti-β-Amyloid (Biolegend, 803014, 1:2000), Rabbit anti-PKM2 (Cell Signaling Technology, 4053, 1:500), Rabbit anti-TSG101 (proteintech, 28283-1-AP, 1:500) for 24 h at 4 °C. .. Brain slices were rinsed 3 times for 10 min with PBS and incubated with appropriate fluorescence-conjugated secondary antibodies, including: Donkey anti-Goat 488 (Jackson, 705-545- 147), Donkey anti-Rabbit 488 (Jackson,711-545-152), Donkey anti-Mouse 555 (Bioss, bs-0295D-BF555) for 2 h at room temperature.



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